Imaging:Article Title: Dynamic fibroblast-immune interactions shape wound healing after brain injury
Article Snippet: .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti- α SMA (Abcam ab5694, 1:300) rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), rabbit anti-Collagen 6 α 1 (Novus Biologicals NB120-6588, 1:200), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3 ε (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000). .. For marmoset imaging, rabbit anti-COL6 (Abcam ab6588, 1:500) was used.
Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz).
Article Title: The emerging fungal pathogen Candida auris induces IFNγ to colonize mammalian hair follicles
Article Snippet: .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:400), rabbit anti-dsRed (Takara 632496, 1:400), rabbit anti-Venus (Thermo OSE00002W, 1:400), goat anti-RFP (Rockland, 200-101-379, 1:400), rabbit anti-zsGreen (Takara, 632474, 1:400), rat anti-CD49f AF647 (Biolegend, 313610, 1:200), rat anti-CD49f AF488 (Biolegend, 313608, 1:200), rat anti-alpha Smooth Muscle Actin (Biotium, BNC040665, 1:100), rabbit anti-Stat1 (Cell Signaling Technology, 14994T, 1:200), rabbit anti-Zo1 (Invitrogen, 40-2200, 1:200), 9661T, 1:200), rabbit anti-PGP9.5 (Abcam, ab108986, 1:200), rabbit anti-B2Microglobulin (Proteintech, 13511-1-AP, 1:200), goat anti-FABP5 (R&D, AF1476-SP, 1:400), rabbit anti- Candida albicans (Abcam, ab252746, 1:200). .. Secondary antibodies were used as necessary at protocol-specific concentrations, conjugated to A488, A555, AF594, and A647 (Life Technologies, Thermo-Fisher).
Article Title: Lung adventitial fibroblasts support type 2 Tregs to shape the response to influenza infection
Article Snippet: Sections were then mounted and imaged with a Leica SP8 confocal microscope. .. Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), rat anti-ER-TR7 (Novus Biologicals NG100-64932), goat anti-Pi16 (R&D Systems AF4929), rabbit anti-proSP-C (Millipore AB3786), goat anti-IL-33 (R&D Systems AF3626), and rat anti-EpCAM (BD Pharmingen 552370). .. Directly conjugated antibodies were also used including mouse anti-αSMA AF488 (eBioscience 53-9760-82), anti-αSMA AF405 (R&D Systems IC1420V025), rat anti-CD45 BV421 (BioLegend 103134), rat anti Lyve1 ef660 (eBioscience 50-0443-82) and rat anti-CD45 (BioLegend 103124).
Chromatin Immunoprecipitation:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz).
Flow Cytometry:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz).
Magnetic Resonance Imaging:Article Title: Dynamic fibroblast-immune interactions shape recovery after brain injury.
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). ..
Article Title: Dynamic fibroblast–immune interactions shape recovery after brain injury
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz). .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies used for murine imaging include chicken anti-GFP (Aves Labs GFP-1020, 1:200), rabbit anti-dsRed (Takara 632496, 1:300), chicken anti-GFAP (Invitrogen PA1-10004, 1:200 or 1:500), rat anti-GFAP (2.2B10, Invitrogen 13-0300, 1:200), rat anti-ER-TR7 (Novus Biologicals NB100-64932, 1:200), rabbit anti-aSMA (Abcam ab5694, 1:300), rat anti-CD31 (MEC13.3, Biolegend 102514, 1:200), goat anti-Desmin (GenWay Biotech GWB-EV0472, 1:200), rat anti-PDGFRb (APB5, Invitrogen 14-1402-82, 1:500), rabbit antiNG2 (Millipore Sigma ab5320, 1:200), goat anti-Decorin (Novus Biologicals AF1060, 1:200), goat anti-Collagen 1 (Southern Biotech 1310-01, 1:500), rabbit anti-Collagen 6a1 (Novus Biologicals NB120-6588, 1:200 or 1:500), rat anti-Periostin (345613, Novus Biologicals MAB3548, 1:200), rat anti-ICAM1 (YN1/1.7.4, Biolegend 116110, 1:200), syrian hamster-anti-CD3e (500A2, BD Biosciences 553238, 1:200), goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91, 1:200), rabbit anti-Iba1 (Aif3, Fujifilm Wako 019-19741, 1:200-1:1000), mouse anti-FGF13 (N235/22, Invitrogen MA5-27705, 1:100), goat anti-CD80 (R&D Systems AF740, 1:200), goat anti-ALPL (Novus Biologicals AF2910, 1:50), rabbit anti-LAMA1 (EPR27258-37, Abcam ab307542, 1:200), rabbit anti-SEMA3C (Invitrogen PA5-103168, 1:100), rabbit anti-CDH18 (Invitrogen PA5-112902, 1:50), rabbit anti-ALDH1A2 (Novus Biologicals NBP2-92915, 1:200), goat anti-SOX10 (R&D Systems AF2864, 1:300), rabbit anti-ASPA (Genetex GTX113389, 1:1000), mouse anti-E-Cadherin (Clone 36, BD Biosciences 610181), rat anti-I-A/I-E (MHCII, M5/114.15.2, eBioscience 14-5321-82), mouse anti-Ly76 (TER119, Biolegend 116232, 1:200), goat anti-mouse IgM (Invitrogen 31172, 1:200), and rabbit anti cleaved Caspase 3 (Cell Signaling Technology 9661T, 1:400; Cell Signaling Technology 9991, Histowiz).
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